EnergyStorm - Human macrophage scavenger receptors: Primary structure, expression, and localization in atherosclerotic lesions

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Human macrophage scavenger receptors: Primary structure, expression, and localization in atherosclerotic lesions

Authors: Matsumoto, Akiyo Itakura, Hiroshige Kodama, Tatsuhiko (Univ. of Tokyo (Japan) National Inst. of Health and Nutrition, Tokyo (Japan)) Naito, Makoto Takahashi, Kiyoshi (Kumamoto Univ. (Japan)) Ikemoto, Shinji Asaoka, Hitoshi Hayakawa, Ikuho (National Inst. of Health and Nutrition, Tokyo (Japan)) Kanamori, Hiroshi Takaku, Fumimaro (Univ. of Tokyo (Japan)) Aburatani, Hiroyuki (Univ. of Tokyo (Japan) Massachusetts Inst. of Tech., Cambridge, MA (United States)) Suzuki, Hiroshi Kobari, Yukage Miyai, Tatsuya (Chugai Pharmaceutical, Tokyo (Japan)) Cohen, E.H. Wydro, R. (Genzyme Corp., Framingham, MA (United States)) Housman, D.E. (Massachusetts Inst. of Tech., Cambridge (United States))

Abstract: Two types of cDNAs for human macrophage scavenger receptors were cloned from a cDNA library derived from the phorbol ester-treated human monocytic cell line THP-1.^The type I and type II human scavenger receptors encoded by these cDNAs are homologous (73% and 71% amino acid identity) to their previously characterized bovine counterparts and consist of six domains: cytoplasmic (I), membrane-spanning (II), spacer (III), α-helical coiled-coil (IV), collagen-like (V), and a type-specific C-terminal (VI).^The receptor gene is located on human chromosome 8. The human receptors expressed in CHO-K1 cells mediated endocytosis of modified low density lipoproteins.^Two mRNAs, 4.0 and 3.2 kilobases, have been detected in human liver, placenta, and brain.^Immunohistochemical studies using an anti-peptide antibody which recognizes human scavenger receptors indicated the presence of the scavenger receptors in the macrophages of lipid-rich atherosclerotic lesions, suggesting the involvement of scavenger receptors in atherogenesis.