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On the mechanism of cell internalization of chrysotile fibers: An immunocytochemical and ultrastructural study
Authors:
Malorni, W.
Iosi, F.
Falchi, M.
Donelli, G. (Ist. Superiore di Danita, Rome (Italy))
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| Abstract: Human breast carcinoma cells (CG5) and human laryngeal carcinoma cells (HEp-2) were exposed to 10 and 50 μml (about 5 μm) chrysotile asbestos fibers.^Morphological and ultrastructural changes were evaluated by means of immunocytochemistry and by scanning and transmission electron microscopy.^The authors attention was focused on the mechanisms of cell internalization and on transport of chrysotile fibers.^The fibers appeared to penetrate the cell cytoplasm and to be translocated in proximity of the nucleus.^Small chrysotile fibers could also be found inside the nucleus of interphase cells.^Involvement of the main cytoskeletal components, i.e., microfilaments, intermediate filaments, and microtubules, in the cytotoxicity of chrysotile fibers was also evaluated.^Their findings suggest that after fiber penetration, a rearrangement of the cytoskeletal apparatus occurs.^It has also been observed that small fibers remain associated with the cytoskeletal framework, which can thus play a role in asbestos intracytoplasmic translocation in epithelial cells.^Furthermore, after the cell has completely recovered its morphology, fiber internalization ultimately seems to lead to the formation of giant multinucleated cells.^These data could be indicative of an interaction occurring between asbestos fibers and the normal mitotic process. |
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| Publication Date: |
01 Aug 1990
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| Resource Type: |
Journal Article
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| Resource Relation: |
Environmental Research ; Vol/Issue: 52:2
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| Country of Publication: |
United States
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| Language: |
English
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Keywords relating to this report: 