Physics Nuclear Physics And Radiation Physics

Quantum-Dot-Based Electrochemical Immunoassay for High-Throughput Screening of the Prostate-Specific Antigen

Authors: Wang, Jun Liu, Guodong Wu, Hong Lin, Yuehe
 
Abstract: In this paper, we demonstrate an electrochemical high-throughput sensing platform for simple, sensitive detection of PSA based on QD labels. This sensing platform uses a microplate for immunoreactions and disposable screen-printed electrodes (SPE) for electrochemical stripping analysis of metal ions released from QD labels. With the 96-well microplate, capturing antibodies are conveniently immobilized to the well surface, and the process of immunoreaction is easily controlled. The formed sandwich complexes on the well surface are also easily isolated from reaction solutions. In particular, a microplate-based electrochemical assay can make it feasible to conduct a parallel analysis of several samples or multiple protein markers. This assay offers a number of advantages including (1) simplicity, cost-effectiveness, (2) high sensitivity, (3) capability to sense multiple samples or targets in parallel, and (4) a potentially portable device with an SPE array implanted in the microplate. This PSA assay is sensitive because it uses two amplification processes: (1) QDs as a label for enhancing electrical signal since secondary antibodies are linked to QDs that contain a large number of metal atoms and (2) there is inherent signal amplification for electrochemical stripping analysis�preconcentration of metal ion onto the electrode surface for amplifying electrical signals. Therefore, the high sensitivity of this method, stemming from dual signal amplification via QD labels and pre-concentration, allows low concentration levels to be detected while using small sample volumes. Thus, this QD-based electrochemical detection approach offers a simple, rapid, cost-effective, and high throughput assay of PSA.
Publication Date: 01 Jan 2008
Report numbers: PNNL-SA-56364
DOE Contract number: AC05-76RL01830
Resource Type: Journal Article
Resource Relation: Journal: Small, 4(1):82-86; Journal Volume: 4; Journal Issue: 1
Research Organizations: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Organizations: USDOE
Country of Publication: United States
Language: English
Keywords relating to this report:
AMPLIFICATION
ANTIBODIES
ANTIGENS
ATOMS
DETECTION
ELECTRODES
IMMUNOASSAY
PROTEINS
SENSITIVITY
SOLAR PROTONS
TARGETS

Subjects:
Related subjects:
ENVIRONMENTAL MOLECULAR SCIENCES LABORATORY
IMMUNOASSAY
PROSTATE-SPECIFIC ANTIGEN