Suppression of deregulated c-MYC expression in human colon carcinoma cells by chromosome 5 transfer
Authors:
Rodriguez-Alfageme, C.
Astrin, S.M. (Fox Chase Cancer Center, Philadelphia, PA (United States))
Stanbridge, E.J. (Univ. of California, Irvine (United States))
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| Abstract: Two-thirds of sporadic colon carcinomas express elevated levels of the c-MYC protooncogene.^In addition, most colon carcinoma cell lines show constitutive elevated expression (10- to 40-fold over normal) of MYC RNA and protein that is not modulated in response to a mitogenic stimulus.^Indirect immunofluorescence has been used to detect c-MYC protein in such cell lines, in hybrid cells resulting from fusions of such lines with cells that regulate MYC normally, and in carcinoma cells to which a normal copy of chromosome 5 has been transferred by microcell fusion.^The deregulated expression of c-MYC is suppressed by fusion with a cell that regulates MYC normally.^In addition, transfer chromosome 5 by microcell fusion results in suppression of deregulated expression.^Suppressed cells are not longer tumorigenic in nude mice.^Loss of the transferred chromosome results in reexpression of the tumorigenic phenotype and in constitutive elevated expression of MYC.^These data indicate that function of a tumor-suppressor gene on chromosome 5 is necessary for the regulated expression of MYC in at least some colon cells.^Loss of this suppressor results in deregulated MYC expression and is a necessary, but most likely not sufficient, event for the expression of the tumorigenic phenotype in a subset of colon carcinomas. |
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| Publication Date: |
15 Feb 1992
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| Resource Type: |
Journal Article
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| Resource Relation: |
Proceedings of the National Academy of Sciences of the United States of America ; Vol/Issue: 89:4
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| Country of Publication: |
United States
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| Language: |
English
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